Once the lights are turned off the image will glow with a light greenish color. The less light in a room the better the glow will be. The glow will eventually fade until the print is exposed to light again.
Glow in the dark inks work on white or colored shirts. The ink is available already mixed and ready for printing. As with any special type of printing you do, always do a wash test to check for a full cure and see how the ink will hold up before your order is received by the customer.